Catalog Number - 68508-10P-3M

Cogent RP C8™ Wide Pore HPLC Columns are designed for reversed-phase separations of larger molecules that require enhanced pore accessibility and efficient mass transfer. The stationary phase consists of a traditional octyl (C8) bonded ligand attached to ultra-high purity silica and is fully double end capped to minimize secondary interactions while providing reproducible retention, excellent peak shape, and long column life. The 300 Å wide pore structure allows proteins, peptides, and other higher molecular weight compounds to access more of the bonded surface area, making this phase particularly suitable for biomolecule analyses and challenging large-molecule separations.

68508-10P-3M is packed with 5 µm RP C8™ Wide Pore particles featuring a 300 Å pore size in a 4.6 mm ID x 100 mm length format. The 100 mm column length provides greater resolving power than a 50 mm column while maintaining efficient run times and moderate solvent consumption. The wide pore architecture improves accessibility and mass transfer for proteins, peptides, and long-chain fatty acids that may be partially excluded from conventional 100 Å media. The RP C8™ chemistry provides lower hydrophobic retention than comparable C18 phases while maintaining excellent selectivity for hydrophobic and moderately non-polar compounds.

This column size is often selected when a 50 mm column does not provide sufficient resolution but longer columns would unnecessarily increase analysis time. The 4.6 mm ID format operates efficiently on conventional HPLC systems and typically performs best at flow rates of approximately 1.0-2.0 mL/min. The balance of resolution, speed, and broad instrument compatibility makes this column a popular choice for analytical, pharmaceutical, biopharmaceutical, and method development laboratories.

Key Benefits

• RP C8™ Wide Pore stationary phase

• Octyl (C8) bonded phase

• Fully double end capped

• Ultra-high purity silica support

• 300 Å wide pore design

• Ideal for proteins and peptides

• Increased resolution compared to 50 mm columns

• Lower retention than C18 phases

• Low backpressure with 5 µm particles

• Compatible with standard HPLC systems

Typical Applications

• Reversed-phase HPLC

• Protein analysis

• Peptide analysis

• Biomolecule separations

• Long-chain fatty acid analysis

• Pharmaceutical analysis

• Biopharmaceutical analysis

• Method development

• Quality control testing

• Routine analytical separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: RP C8™ Wide Pore

• Chromatography Mode: Reversed Phase

• Bonded Phase: Octyl (C8)

• Endcapping: Fully Double End Capped

• Particle Size: 5 µm

• Pore Size: 300 Å

• Column Dimensions: 4.6 mm ID x 100 mm

• Recommended Flow Rate: 1.0-2.0 mL/min

• Column Hardware: Stainless Steel

• Brand: Cogent

Important Notes

• Designed for large molecule reversed-phase separations

• 300 Å pores enhance accessibility for proteins, peptides, and biomolecules

• Provides lower retention than comparable C18 columns

• Suitable for hydrophobic and moderately non-polar compounds

• Excellent choice for peptide analysis, protein analysis, and long-chain fatty acid separations

• 100 mm length provides greater resolving power than shorter analytical columns

• 5 µm particles provide low backpressure and broad instrument compatibility

• Compatible with standard HPLC systems operating at conventional analytical flow rates

• Frequently selected when improved resolution is needed without substantially increasing run times

• Manufactured using ultra-high purity silica and fully double end capped bonding technology